Protein Method Blocking Buffers
LICOR Intercept T20 (TBS) Antibody Diluent (10 x 500 mL)
Intercept T20 (TBS) Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects. The diluent contains Intercept (TBS) Blocking Buffer in Tris-buffered saline (TBS) preformulated with Tween 20. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (TBS) Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
Biotium TrueBlack IF Background Suppressor System (Permeabilizing)
The TrueBlack Background Suppressor System (Permeabilizing) is a buffer system designed for blocking background from both non-specific antibody binding as well as direct interaction of fluorescent dyes on antibodies with cells or tissue sections.
Boca Scientific Inc The Blocking Solution, 500ml
The Blocking Solution is a high-performance blocking reagent designed for saturating free binding sites on plastic surfaces or other protein binding surfaces and for minimizing nonspecific binding to surfaces in immunoassays. It is ideal for assays that require high reproducibility and low coefficients of variation, such as assays for pre-clinical and clinical studies, production of high quality diagnostics or measurement of precious specimens. The Blocking Solution is based on highly purified casein that has been chemically modified for maximum adsorption efficiency and a dense blocking layer. By incubating a surface with The Blocking Solution, such as a microtiter plate or western blotting membrane, free spaces on the surface are saturated by the components of this blocker. This minimizes unwanted binding of the analyte or the detection antibody to the surface, avoiding background and improving sensitivity of the assay.
SCANTIBODIES LABORATORY INC HETEROPHILIC BLOCKING TUBE (3IX762) 50PK
The 3IX762 HBT (Heterophilic Blocking Tube) from Scantibodies Laboratory, Inc. contains a unique blocking reagent composed of specific binders which inactivate heterophilic antibodies. Once the specific binders have bound to the heterophilic antibodies, the antibodies are no longer able to cause immunoassay interference. The reagent is in the form of a lyophilized pellet at the bottom of the tube and each tube contains enough reagents to inactivate the heterophilic antibodies in 500 μl of sample material. The HBT also allows for the rapid and simple elimination of false positive heterophilic interference in plasma or serum for immunoassays (i.e., FSH, LH, Prolactin, TSH, Ferritin, CEA, AFP, hCG, HBsAg, CK-MB, CA 125, CA 19-9, NSE, etc.).
Kd Medical Inc PBS 20X + TWEEN 20, 1 L
Concentrated 20X PBS and Tween 20 used to be diluted to a 1X working solution. Used as a wash buffer in various laboratory applications. One 1 Liter bottle.
Bioworld Casein-Blocking Buffer in PBS w/ Azide, 500 mL
Product DescriptionCasein-blocking buffer is 3% in PBS with 0.02% sodium azide. It is a liquid stored between temperatures of -30°C to -10°C, and is used for Western blot and ELISA. Buffer used for blocking excess binding sites on membranes and microplates in antibody-based detection methods.
Bioworld Casein-Blocking Buffer in TBS, 1 L
Casein Blocking Buffer in TBS Blot blocking buffer used to block non-specific binding site for antibodies on the membrane. Technical Information: 1% Casein in TBS
SCANTIBODIES LABORATORY INC HBR 1, PURIFIED (3KC533) 1.0G
The 3KC533 Heterophilic Blocking Reagent 1, Purified from Scantibodies Laboratory, Inc. contains specific murine immunoglobulins that block the heterophilic interaction by active binding to the heterophilic antibodies, which are capable of cross-linking the capture and the detection antibodies used in the immunoassay, resulting in false positive readings. The attachment of HBR-1 to the heterophilic antibodies blocks this cross-linking, and eliminates the interference caused by the heterophilic antibodies in the humoral fluids. In addition to its active blocking, this product is also characteristic of its passive blockage of the heterophilic interaction as well. The HBR is a liquid reagent with a protein concentration of 20 ± 2 mg/ml. The immunoglobulins are dissolved in a phosphate buffer with a pH of 7.2-7.6. The immunoglobulins in this product are at a purity of greater than or equal to 95%.
Alkali Scientific Dry Powder Milk, 100 G
Dry Powder Milk, 100 G, is a non-fat milk powder used primarily in microbiological culture media and as a nutrient source in laboratory applications. It is commonly employed in the preparation of media for the growth of microorganisms, particularly those requiring a dairy-based nutrient source. The powder is utilized in various applications, including culturing bacteria and fungi, and in fermentation processes. This 100g package size is ideal for small-scale culture media preparation, ensuring precise control over the concentration of nutrients used in experiments. The product is highly versatile and essential in the preparation of high-quality, reproducible media formulations in research laboratories.
Alkali Scientific PBS/Tween 20 10X Solution, 500 Milliliters
PBS/Tween 20 10X Solution is a concentrated buffer solution that combines the benefits of phosphate-buffered saline with Tween 20, a non-ionic detergent, to enhance the solubilization of biological molecules. This 500mL solution is commonly used for washing and blocking steps in immunological assays, particularly in enzyme-linked immunosorbent assays (ELISA) and western blotting. The addition of Tween 20 helps to reduce non-specific binding and improve the sensitivity of the assay. The 10X concentration allows for easy dilution to working strength, making this product versatile for various laboratory applications. It is an essential reagent for researchers working with proteins, antibodies, and cell cultures.
Alkali Scientific Dry Powder Milk, 500 G
Dry Powder Milk, 500 G, is a high-quality non-fat milk powder that is commonly used in microbiology and biochemical research. It serves as a nutrient source in the preparation of culture media for growing microorganisms such as bacteria and fungi. The milk powder is particularly important for experiments that involve fermentation, microbial cultivation, and the study of metabolic processes. It is often used in laboratories to prepare nutrient-rich media for routine microbiological testing, ensuring the optimal growth of a wide range of microbial species. The 500g size is ideal for smaller-scale media preparation and can be easily incorporated into custom laboratory formulations.
Research Products International Corp TBS with Tween, 20X, 7.4 pH, Sterile Buffered, 1 Liter
TBS with Tween, 20X, 7.4 pH, Sterile Buffered is a commonly used buffer solution in the life science marketplace. TBS stands for Tris-Buffered Saline, and Tween refers to Tween 20, a nonionic detergent.
TBS with Tween is primarily used in various applications such as Western blotting, immunohistochemistry, and antibody-based assays. It serves as a buffer for sample dilution, antibody washing, and blocking steps during these experimental procedures.
The buffer solution is prepared at a 20X concentration, meaning it is twenty times more concentrated than the final desired concentration. This concentrated form allows for efficient storage and transportation.
TBS with Tween, 20X, 7.4 pH, Sterile Buffered is specifically formulated to have a pH of 7.4, which is the physiological pH of many biological systems.
LICOR Intercept T20 Protein-Free Antibody Diluent (3x500mL)
Intercept T20 (TBS) Protein-Free Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects. The diluent contains Intercept (TBS) Protein-Free Blocking Buffer in Tris-buffered saline (TBS) preformulated with Tween 20. This diluent can be used where animal-based products are prohibited. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (TBS) Protein-Free Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
INNOVEX BIOSCIENCES UNIVERSAL Fc RECEPTORS BLOCKER 4X-CONCENTRATE 15 ML
Fc Receptor Blocker (4x Concentrate)